Changes in c‐erbB‐2 Immunoexpression in Feline Endometrial Adenocarcinomas          下载免费PDF全文

AL Saraiva  R Payan‐Carreira  F Gärtner  F Faria  LM Lourenço  MA Pires 《Reproduction in domestic animals》2016,51(1):33-39

Human epidermal growth factor receptor type 2 (c‐erbB‐2), an oncoprotein with potential prognostic marker and therapeutic use, is overexpressed in several human and animal tumours. But information regarding this molecule in feline tumours is scarce. This study aimed to assess the changes in the immunohistochemical expression of c‐erbB‐2 in feline endometrial adenocarcinomas (FEA) compared to normal endometrium. An immunohistochemistry assay using a specific antibody against c‐erbB‐2 was performed in FEA samples (n = 34) and in normal endometrium in the follicular (FS; n = 12) and luteal (LS; n = 11) stages. In FEA, the c‐erbB‐2 immunoexpression was assessed in neoplastic epithelial cells whilst in normal endometria it was individually evaluated in the surface and the superficial and deep glandular epithelia (SE, SGE and DGE, respectively). In FS and in LS, all the epithelia were positive for c‐erbB‐2; positivity was higher in the SE and the SGE than in DGE. Twenty of the 34 FEA samples (58.8%) were positive for c‐erbB‐2 immunolabelling. Nevertheless, its expression was higher in all the epithelia in the FS compared to FEA (p ≤ 0.0001) or the LS (p = 0.016). The results presented herein suggest that c‐erbB‐2 molecule is differently expressed in the feline endometrium through the oestrous cycle and though it may also be involved in feline endometrial carcinogenesis, a question remains unanswered on the importance of additional pathways of epithelial proliferation in the neoplastic changes in feline endometrium.  相似文献   

Osteosarcoma tissues and cell lines from patients with differing serum alkaline phosphatase concentrations display minimal differences in gene expression patterns          下载免费PDF全文

L. C. de Sá Rodrigues  K. E. Holmes  V. Thompson  C. M. Piskun  S. E. Lana  M. A. Newton  T. J. Stein 《Veterinary and comparative oncology》2016,14(2):e58-e69

Serum alkaline phosphatase (ALP) concentration is a prognostic factor for osteosarcoma in multiple studies, although its biological significance remains incompletely understood. To determine whether gene expression patterns differed in osteosarcoma from patients with differing serum ALP concentrations, microarray analysis was performed on 18 primary osteosarcoma samples and six osteosarcoma cell lines from dogs with normal and increased serum ALP concentration. No differences in gene expression patterns were noted between tumours or cell lines with differing serum ALP concentration using a gene‐specific two‐sample t‐test. Using a more sensitive empirical Bayes procedure, defective in cullin neddylation 1 domain containing 1 (DCUN1D1) was increased in both the tissue and cell lines of the normal ALP group. Using quantitative PCR (qPCR), differences in DCUN1D1 expression between the two groups failed to reach significance. The homogeneity of gene expression patterns of osteosarcoma associated differing serum ALP concentrations are consistent with previous studies suggesting serum ALP concentration is not associated with intrinsic differences of osteosarcoma cells.  相似文献   

Importance of secondary damage in downer cows          下载免费PDF全文

PJ Poulton  AL Vizard  GA Anderson  MF Pyman 《Australian veterinary journal》2016,94(5):138-144

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Supplementation with sunflower seeds in beef cattle did not impact on oocyte and in vitro embryo production          下载免费PDF全文

AL Baltazar  GM de Mattos  BM Ropelli  SMG Firetti  C Castilho  G Pugliesi  MBC Maldonado  M Binelli  JOF Silva  GC Lupatini  BS Lafuente  CMB Membrive 《Reproduction in domestic animals》2018,53(3):801-808

Supplementation with compounds rich in linoleic acid, including sunflower seed supplementation, promotes increase in conception rates in cows. We aimed to evaluate whether the sunflower seed (linoleic acid source) supplementation in beef donor females alters the plasma concentrations of cholesterol, triglycerides, HDL and LDL, increases the number and quality of oocytes, increases the cleavage rates and determines an improvement in number and quality of in vitro produced blastocysts. Thus, Nelore females were divided into two groups of 15 animals to receive supplementation with or without sunflower seed for 57 days. Females underwent follicular aspiration and the oocytes were subjected to in vitro embryo production. There was no difference (p > .1) between control group and group supplemented with sunflower seed on the number of displayed follicles; number of aspired oocytes; recovery rate; cleavage rate; number of embryos; number of blastocysts; embryos number of grades I and II; plasma concentrations of total cholesterol, triglycerides; HDL and LDL. Therefore, sunflower seed supplementation in oocyte donors did not increase the number and quality of oocytes, cleavage rates and the number and quality of blastocysts produced in vitro.  相似文献   

Study of the effect of genotype–environment interaction on age at first calving and production traits in Nellore cattle using multi‐trait reaction norms and Bayesian inference          下载免费PDF全文

Francisco Ribeiro de Araujo Neto  Newton Tamassia Pegolo  Rusbel Raul Aspilcueta‐Borquis  Matilde Conceição Pessoa  Alexandre Bonifácio  Raysildo Barbosa Lobo  Henrique Nunes de Oliveira 《Animal Science Journal》2018,89(7):939-945

This study investigated the effects of genotype–environment interaction on yearling weight, age at first calving and post‐weaning weight gain in Nellore cattle using multi‐trait reaction norm models. The environmental gradient was defined as a function of the mean yearling weight of the contemporary groups. A first‐order random regression sire model with four classes of residual variance was used in the analyses and Bayesian methods were applied to estimate the (co)variance components. The heritability estimates ranged from 0.284 to 0.547, 0.222 to 0.316 and 0.256 to 0.522 for yearling weight, age at first calving and post‐weaning weight gain, respectively. The lowest genetic correlations between environment groups for each trait were 0.38, 0.02 and 0.04 for yearling weight, age at first calving and post‐weaning weight gain, respectively. Differences in the correlation estimates were observed between traits in the same environments, with the magnitude of the estimates tending toward zero as the environment improved. The results highlight the importance of including genotype–environment interactions in genetic evaluation programs considering the differences observed between environmental groups not only in terms of heritability, but also of genetic correlations.  相似文献   

THE EFFECTS OF LEVEL OF NITROGEN FERTILIZER ON BEEF PRODUCTION FROM GRAZED PERENNIAL RYEGRASS/WHITE–CLOVER PASTURES     

F. E. Alder    S. J. Cowlishaw  J. E. Newton  D. T. Chambers  † 《Grass and Forage Science》1967,22(3):194-203

The treatments in this summer–grazing experiment were designed as a 3X3 factorial; 3 levels of nitrogenous fertilizer, supplying 0, 336 and 672 kg N/ha per annum, and 3 stocking rates. The fertilizer was applied in 6 to 8 dressings at intervals of 3 to 4 weeks. The animals used were fattening Hereford X Friesian steers. The stocking rates were calculated on the basis of data available on herbage production under N and irrigation treatments and varied according to the level of N input. The experiment was laid out in 6 randomized blocks, which were grazed in rotation. Blocks were removed from the grazing cycle in spring and early summer of each of the 3 years (1962—-4) over which the experiment was conducted and cut for conservation: yields were recorded. Details are given of: grazing management; results of studies of soil–N levels; the quantities of herbage removed for conservation: the quantity, chemical and botanical composition of the herbage available for grazing; and herbage consumption.  相似文献   

Brighter outlook for potatoes     

J. G. Lipman  H. O. Werner  E. P. Miller  Walter M. Peacock  Julian Dimock  R. F. Howard  R. G. Newton  Wm. H. Martin  George H. Valentine  Dee Crane  O. C. Hicks  H. C. Moore 《American Journal of Potato Research》1925,2(6):221-258

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Largemouth bass predators reduce growth,feeding and movement in juvenile channel catfish     

M. L. Fine  M. S. Mayo  S. H. Newton  E. N. Sismour 《Ecology of Freshwater Fish》2011,20(1):114-119

Fine ML, Mayo MS, Newton SH, Sismour EN. Largemouth bass predators reduce growth, feeding and movement in juvenile channel catfish. Ecology of Freshwater Fish 2011: 20: 114–119. © 2010 John Wiley & Sons A/S Abstract – Animals utilise sensory cues to make decisions that will decrease their vulnerability to predation. With a well‐developed olfactory system and taste buds located inside the mouth and on the external body surface, catfishes are excellent subjects to investigate nonconsumptive predator effects. Juvenile channel catfish Ictalurus punctatus are often eaten by largemouth bass Micropterus salmoides, and we tested the hypothesis that largemouth bass fed catfish would adversely affect foraging and growth in channel catfish. Groups of catfish were placed in tanks partitioned by a mesh screen: experimental tanks had a largemouth bass on the other half of the tank, and control tanks had an empty chamber. Experimental catfish exhibited a long‐term decrease in spontaneous motion, feeding and growth. Feeding catfish to the bass caused the experimental catfish to freeze after a multi‐second latency suggesting transport of an alarm cue. Thus, there were long‐term effects from the continuous presence of the bass and additional short‐term cues from the bass consuming a catfish.  相似文献   

Spectrophotometric determination of caffeine in coffee products: collaborative study.     

J M Newton 《Journal of the Association of Official Analytical Chemists》1979,62(4):705-708

An ultraviolet (UV) spectrophotometric method for determining caffeine in regular and decaffeinated coffee products has been studied collaboratively. Nine laboratories participated in this study which compares the proposed UV method with the official AOAC micro Bailey-Andrew method. Caffeine content was determined on as-is basis on 8 samples of green, roasted, and soluble coffees. The coefficients of variation for the proposed method ranged from 2.02 to 6.98% for the 8 samples studied. The results agreed well with those from the Bailey-Andrew Method. The method was adopted as official first action.  相似文献   

An epidemiological overview of the equine influenza epidemic in Great Britain during 2019     

Fleur Whitlock  John Grewar  Richard Newton 《Equine veterinary journal》2023,55(1):153-164

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